Human CD4 is a cell surface glycoprotein of 55 kd expressed on most thymocytes, on about two thirds of peripheral T cells, and on some monocyte macrophage lineage cells. CD4 interacts with MHC Class II molecules in an accessory role during foreign antigen recognition by T cells (3). The extracellular portion of CD4 is an array of four Ig-like domains. The amino terminal region of CD4 is a receptor for the HIV-1 viral protein gp120.

Antibodies QS4120 and M-T441 (cat #148-020) each bind to a distinct epitope of CD4 and are suitable as a matched pair for EIA using QS4120 as capture and Biotinylated M-T441(cat# 148-030) as detector.

Isotype: Murine IgG1

Immunogen: CD4+ transfectant/human CEM

Specificity: Antibody QS4120 recognizes an extracellular epitope in the CDR2-like loop within domain 1 region of the CD4 molecule (6).

Functional Application: Antibody QS4120 will block binding of HIV-1 gp120 protein to CD4 (4) and also blocks HLA Class II Rosette formation.

References:

1. D. Healey, et al, (1990) J Exp Med 172: 1233-1242.

2. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 476-478.

3. R. König, et al, (1995) J Exp Med 182: 779-787.

4. S. Ugolini, et al, (1997) J Immunol 159: 3000-3008.

5. S. Dornan et al, (2002) J Biol Chem 277(3): 1912.

6. S.E.M. Howie, D.J. Harrison, et al. (1999) FASEB 13(8): 503-511. PMID: 10064617.

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