Human CD40 is a member of the tumor necrosis factor (TNF) receptor family and is present on all B cells except plasma cells. CD40 is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 plays an important role in B cell activation and the interaction with its ligand CD154 (CD40 Ligand) is essential for isotype switching (1).

Molecular Structure: A soluble fusion protein consisting of the mature extracellular (173aa) domain of human CD40: epptacrekqylinsqccslcqpgqklvsdctefteteclpcgesefldtwnrethchqhkycdpnlglrvqqkgtsetdtictceegwhctseacescvlhrscspgfgvkqiatgvsdticepcpvgffsnvssafekchpwtscetkdlvvqqagtnktdvvcgpqdrlr

+linker: gt

fused to murine IgG2a Fc (233aa): eprgptikpcppckcpapnllggpsvfifppkikdvlmislspivtcvvvdvseddpdvqiswfvnnvevhtaqtqthredynstlrvvsalpiqhqdwmsgkefkckvnnkdlpapiertiskpkgsvrapqvyvlpppeeemtkkqvtltcmvtdfmpediyvewtnngktelnykntepvldsdgsyfmysklrvekknwvernsyscsvvheglhnhhttksfsrtpg

Predicted monomeric molecular weight of 45.6 kd. The dimeric protein runs at ~95 kD in SDS-PAGE.

Transfectant Cell Line: CHO

Functional Application: CD40-muIg fusion protein blocks binding of anti-human CD40 to Raji human tumor cells.

References:

1. T.A. Foy, et al, (1996) Annu Rev Immunol 14: 591-617.

2. M.E. Ozaki, et al, (1997) J Immunol 159: 214-221

3. N. Hubbard, T. R. Torgerson, et al. (2016) Blood :blood-2015-11-683235; doi: https://doi.org/10.1182/blood-2015-11-683235

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