Human CD95 (APO-1/FAS) is a type I cell surface glycoprotein that is strongly upregulated on activated T cells, B cells, NK cells and thymocytes (1). CD95 plays an important role in programmed cell death or apoptosis (2). Apoptosis appears to be a mechanism for regulating the immune response (3, 4).

Molecular Structure: A soluble fusion protein consisting of the mature extracellular (159 aa) domain of human CD95 fused to a linker (4 aa) and human IgG1 Fc + hinge (232 aa), with a (non glycosylated) predicted monomeric molecular weight of 44.3 kd.

CD95(EC):

rlssksvnaqvtdinskglelrktvttvetqnleglhhdgqfchkpcppgerkardctvngdepdcvpcqegkeytdkahfsskcrrcrlcdeghgleveinctrtqntkcrckpnffcnstvcehcdpctkcehgiikectltsntkckeegsrsnlg

linker: gtyv

Human Ig Fc + hinge:

eprscdkthtcppcpapellggpsvflfppkpkdtlmisrtpevtcvvvdvshedpevkfnwyvdgvevhnaktkpreeqynstyrvvsvltvlhqdwlngkeykckvsnkalpapiektiskakgqprepqvytlppsrdeltknqvsltclvkgfypsdiavewesngqpennykttppvldsdgsfflyskltvdksrwqqgnvfscsvmhealhnhytqkslslspgk

Transfectant Cell Line: CHO

Functional Application: CD95-huIg fusion protein inhibits CD178 mediated apoptosis of Jurkat cells.

References:

1. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford (1995) p. 1142-1148.

2. S. Nagata & P. Golstein (1995) Science 267: 1449-1456.

3. S. Nagata & T. Suda (1995) Immunol Today 16: 39-43.

4. D.H. Lynch, F. Ramsdell & M.R. Alderson (1995) Immunol Today 16: 569-574.

5. H Wajant, (2003) Essays Biochem 39:53-71.

6. A. Ackery, M G Fehlings, (2006) J Neurotrauma 23(5): 604-616.

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